Solution in Summary

Solution Information		help
Enzyme:	Cyclin-dependent kinase 4/G1/S-specific cyclin-D1
inhibitor:	BDBM5655
substrate:	GST-Rb fusion protein

*Solution Type:*	Aqueous
pH at Preparation:	7.2
Temp. Prep.:	30
Comments:	n/a

*Solute 1:*	DTT
Concentration:	1 mM
Concentration:	n/a
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*Solute 2:*	MgCl2
Concentration:	15 mM
Concentration:	n/a
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*Solute 3:*	EGTA
Concentration:	5 mM
Concentration:	n/a
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*Solute 4:*	sodium vanadate
Concentration:	1 mM
Concentration:	n/a
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*Solute 5:*	beta-glycerophosphate
Concentration:	60 mM
Concentration:	n/a
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*Solute 6:*	p-nitrophenyl phosphate
Concentration:	15 mM
Concentration:	n/a
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*Solvent 1:*	water
Concentration:	1.0
Purity:	n/a
Purification Meth.:	n/a
Comments:	n/a

*pH Buffer 1:*	MOPS
Concentration:	25 mM
ΔH_ion:	n/a
Comments:	n/a

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